16S/18S/ITS Amplicon Sequencing-NGS
Siffofin Sabis
● Tsarin tsarin dandali: Illumina NovaSeq.
● Ƙaddamar da ƙananan yankuna na 16S, 18S da ITS, a tsakanin sauran maƙasudin haɓakawa.
● Zaɓuɓɓuka masu sassauƙa na amplicon.
● Kwarewar aikin da ta gabata tare da maƙasudin haɓakawa da yawa.
Amfanin Sabis
●Babu warewa:Gano da sauri na abubuwan ƙwayoyin cuta a cikin samfuran muhalli.
●Babban ƙuduri: A cikin ƙananan abubuwa masu yawa a cikin samfuran muhalli.
●Yadu Dace: Nazarin al'umma daban-daban na ƙananan ƙwayoyin cuta.
●Cikakken Binciken Bioinformatic: Sabbin fakitin QIIME2 (ƙididdigar ƙididdigewa game da ilimin halittu) tare da nazari iri-iri dangane da bayanan bayanai, annotation, OTU/ASV.
●Ƙwararren Ƙwararru: Tare da ayyukan 150 dubun amplicon jerin ayyukan da aka gudanar a kowace shekara, BMKGENE ya kawo shekaru goma na kwarewa, ƙungiyar bincike mai ƙwarewa, cikakkun abun ciki, da kuma kyakkyawan goyon bayan tallace-tallace.
Ƙayyadaddun Sabis
| Laburare | Dabarun Jeri | An ba da shawarar bayanai |
| Amplicon | Farashin PE250 | 50/100/300K tags (Karanta Biyu) |
Bukatun Sabis
| Hankali (ng/µL) | Jimlar adadin (ng) | girma (µL) |
| ≥1 | ≥200 | ≥20 |
● Ƙasa / sludge: 1-2g
● Abun ciki na ciki-dabba: 0.5-2g
● Abubuwan ciki na ciki-kwari: 0.1-0.25g
● Filayen tsire-tsire (ƙasa mai yalwa): 0.1-0.5g
● Fermentation broth wadatar labe: 0.1-0.5g
● Faeces (manyan dabbobi): 0.5-2g
● Faeces ( linzamin kwamfuta): 3-5 hatsi
● Ruwan lavage na alveolar na huhu: takarda tace
● Swab na Farji: 5-6 swabs
● Fatar fata / al'aura swab / miya / nama mai laushi na baki / pharyngeal swab / swab swab: 2-3 swabs
● ƙananan ƙwayoyin cuta: takarda tace
● Ruwa / iska / biofilm: takarda tace
● Endophytes: 1-2g
● Plaque na hakori: 0.5-1g
Gudun Aikin Sabis
Samfurin bayarwa
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
Ya haɗa da bincike mai zuwa:
- Raw data ingancin iko
- OTU clustering / De-noise(ASV)
- Bayanin OTU
- Binciken bambancin Alpha: fihirisa da yawa, gami da Shannon, Simpson da ACE.
- Binciken bambancin beta
- Binciken Inter-group
- Binciken daidaitawa: tsakanin abubuwan muhalli da abubuwan OUT da bambancin
- 16S aikin tsinkayar kwayoyin halitta
Histogram na rarraba taxonomic
Taswirar zafi mai tarin yawa taxonomic
Binciken bambancin Alfa: Rarefaction curve
Binciken bambancin beta: NMDS
Binciken Intergroup: Binciken biomarker LEFSE
Bincika ci gaban da sabis na amplicon na BMKGene ya sauƙaƙe tare da Illumina ta hanyar tarin wallafe-wallafe.
Dong, C. et al. (2022) 'Taro, Core Microbiota, da Aiki na Rhizosphere Soil da Bark Microbiota a Eucommia ulmoides', Frontiers a Microbiology, 13. doi: 10.3389/FMICB.2022.855317/FULL.
Li, Y. et al. (2023) 'Synthetic kwayan cuta consortia dashi don maganin Gardnerella vaginalis-induced kwayan vaginosis a cikin mice', Microbiome, 11 (1), shafi 1-14. doi: 10.1186/s40168-023-01497-y
Yang, J., Fu, Y. da Liu, H. (2022) 'Microbiomes na ƙurar iska da aka tattara yayin gwajin tallafin rayuwa na rufaffiyar ƙasa na tushen "Lunar Palace 365"', Microbiomes Environmental, 17(1), pp. 1-20. doi: 10.1186/S40793-022-00399-0/FIGURES/8.
Yin, S. et al. (2022) 'Yawancin abubuwan da ke dogaro da abinci na kwayoyin aiki masu alaƙa da canjin nitrogen da ke sarrafa asarar nitrogen a cikin takin zamani', Fasahar Bioresource, 361, p. 127678. doi: 10.1016/J.BIORTECH.2022.127678.
