10x Genomics Verial Transcriptome
Tsarin fasaha
Fasas
● Adduri: 100 μm
Tuntu tabo diamita: 55 μm
● Yawan aibobi: 4992
Yankin kama: 6.5 x 6.5 mm
● Kowanne wuraren barbakiya an ɗora su da strimers wanda aka hada da sassan 4:
- poly (dt) wutsiya ga Mista Pringing da CDNA Synthesis
- Musamman mai gano kwayoyin halitta (UMI) don gyara daidaituwar amplification da
- Spatial Barcode
- Inganta jerin searfafa Karanta 1
● H & Eting na sassan
Yan fa'idohu
●Sabis na tsayawa: Haɗin duk ƙwarewar fasaha da matakan fasaha, ciki har da sashe, sequiry, nama, kayan kwalliya, shirye-shiryen ɗakin karatu, kayan kwalliya da kuma manufar.
● babban fasaha mai fasaha: Tare da kwarewa a cikin nau'ikan nama sama da 250 da nau'ikan 200+ waɗanda suka hada da ɗan adam, linzamin kwamfuta, dabbobi masu shayarwa, kifi da tsire-tsire.
●Sabuntawar lokaci akan duka aikin: Tare da cikakken iko na ci gaban gwaji.
●Cikakken daidaitattun daidaito:Kunshin ya ƙunshi nazarin 29 da adadi mai inganci.
●Bincike na bayanai da kuma gani: Akwai don buƙatun bincike daban-daban.
●Zaɓin Binciken haɗin haɗin gwiwar tare da sel Mrna Sequining
Muhawara
| Bukatun Samfura | Leburare | Tsarin Sequining | Bayanai da shawarar | Iko mai inganci |
| Samfuran cry-act (Mafi kyawun diamita: Kimanin lamba 6x6x6 mm³) 2 Tubalan kowane samfurin | 10x Vifium CdNA Laburare | Illumina Pe150 | 50k pe karanta kowane wuri (60GB) | Rin> 7 |
Don ƙarin cikakkun bayanai game da shirye-shiryen yin shiri da aikin motsa jiki, don Allah jin daɗin magana daMasanin BMkgene
Fiɗa sabis
A cikin tsarin shiri na samfurin, ana yin gwajin hakar RNK don tabbatar da ingancin RNA mai inganci. A cikin matakin ingantawa nama an murƙushe sassan kuma an hango su kuma yanayin permabililation ga sakin Misna daga nama daga nama daga nama daga nama. Ana amfani da yarjejeniya ta yarjejeniya yayin aikin ɗakin karatu, da kuma binciken kayan rubutu.
Cikakken aikin motsa jiki ya ƙunshi sabuntawar lokaci da kuma tabbacin abokin ciniki don kula da madauki mai amsa, tabbatar da kisan mai santsi.
Ya hada da bincike mai zuwa:
Kayyade ingancin bayanai:
o fitarwa na bayanai da kuma rarrabawa iri
o gano Gene a kowane wuri
o murfin nama
Bincike na ciki:
o Ingancin Gene
o tabo cuntiting, ciki har da rage rage yawan bincike
o Musanya daban-daban na tantancewa tsakanin gungu: shaidar alamomin
o An bayyana ambaliyar aiki da wadatar da alamun alamun
Binciken Inter-Stration
o sake haduwa da aibobi daga samfuran biyu (misali da cuta da sarrafawa) kuma sake cluster
o Shaida shi na alamomin ramuka ga kowane tari
o An bayyana ambaliyar aiki da wadatar da alamun alamun
o Quesididdigar bayyana iri ɗaya tsakanin kungiyoyi
Bincike na ciki
Tabo clustering
Marker ya samar da tantanin ganewa da rarraba spatial
Binciken Inter-Stration
Hadewar bayanai daga duka kungiyoyin da sake sake cluster
Alamar jakunkuna na sabon gungu
Bincika cigaban da aka yi masa ƙaddamar da Tratial ta Bmkgene ta Bmkgene a cikin wadannan vissium a cikin wadannan littattafan da aka nuna:
Chen, D. et al. (2023) 'MTHL1, mai yiwuwa Drosophiuuue ya dace da Drosophize na Mamma GPCRRS, yana cikin kashin kansa a cikin tsoffin halayen sel a kwari',Ayyukan Kwalejin Kasa na Kimiyya ta Amurka ta Amurka, 120 (30), p. E2303462120. Doi: /10.1073/Pnas.230346210
Chen, Y. et al. (2023) 'Karfe yana ba da damar ɗaukar ƙaho na ƙuduri na bayanan ɓulawa na spatiotomptomicTriefings a cikin Bioinformatics, 24 (2), PP. 1-10. Doi: 10.1093 / BIC / BBAD068.
Liu, C. et al. (2022) 'Atlas Atlas na Orgogogenesis a cikin Furannin furanni na Origid',Binciken nucleic acid, 50 (17), PP. 9724-9737. Doi: 10.1093 / nar / gkac773.
Wang, J. et al. (2023) 'Haɗewar fassarar fassarar bayanan sirri da nucleus RNA na RNA ya bayyana dabarun warkewa don kawar da ƙwayar cuta don Uterine Leiyyoma',Journal na kasa da kasa game da kimiyyar halittu, 19 (8), PP. 2515-2530. Doi: 10.7150 / ijbs.83510.
