Congratulations! In January 2025, One client of BMKGENE published a research paper titled “Spatiotemporal dynamics of early oogenesis in pigs” in the academic journal Genome Biology. This study utilized single-cell RNA sequencing and spatial transcriptomics technologies to dissect the gene expression and spatial organization characteristics during early oogenesis in pigs. The research discovered that porcine germ cells are distributed in a “cortex-to-medulla” pattern within the ovary, and the process of oogenesis is similar to that in humans, indicating that pigs are excellent models for studying human oogenesis. RNA velocity analysis revealed the characteristics of granulosa cell lineages. Spatial co-localization analysis and cell-cell communication analysis demonstrated that the communication patterns between germ cells and somatic cells in the cortical and medullary regions are different.
Notably, the NOTCH signaling pathway and ECM proteins play crucial roles in regulation, highlighting the significance of the ovarian microenvironment in determining germ cell fate. These findings not only enrich our understanding of the complex mechanisms underlying the formation of porcine reproductive traits but also provide new perspectives and methods for research in the field of reproductive medicine.
BMKGENE provided BMKMANU S1000 spatial transcriptomics technical services for the E65 embryonic ovaries in this study.
If you would like to learn more about this study, access this link. For more information on our sequencing and bioinformatics services, you can talk to us here.
Post time: Feb-20-2025