Eukaryotic mRNA Sequencing-NGS
Features
● Optional directional mRNA library preparation to enable obtaining strand-specific sequencing data
● Different BI analysis cover all our clients needs
Advantages
● Extensive Expertise: We have processed over 600,000 samples, spanning diverse sample types such as cell cultures, tissues, and body fluids. We bring the wealth of expertise to every project.
● Rigorous Quality Control: We implement core control points across all stages, from sample preparation to library preparation, sequencing and bioinformatics. Our meticulous monitoring ensures the delivery of consistently high-quality results.
● Comprehensive Annotation: We use multiple databases to functionally annotate the Differentially Expressed Genes (DEGs) and perform corresponding enrichment analyses. This comprehensive approach provides insights into the cellular and molecular processes underlying the transcriptome response, ensuring you get all the information possible about your experiment’s data.
● Post-Sales Support: We understand how important is to be present, that’s why our commitment extends beyond project completion with a 3-month after-sale service period. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
Sample Requirements and Delivery
| Library | Sequencing strategy | Data recommended | Quality Control |
| Poly A enriched |
Illumina PE150 DNBSEQ-T7 |
6 -10 Gb |
Q30≥85% |
Sample Requirements:
Nucleotides:
Mimimum Sample Requirements:
|
Conc.(ng/μl) |
Amount (μg) |
Purity |
Integrity |
|
|
Standard Library |
≥ 10 |
≥ 0.2 |
OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥4.0; For animals: RIN≥4.5; 5.0≥28S/18S≥1.0; limited or no baseline elevation |
|
Directional Library |
≥ 10 |
≥ 0.2 |
OD260/280=1.7-2.5 OD260/230=0.5-2.5 Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥4.0; For animals: RIN≥4.5; 5.0≥28S/18S≥1.0; limited or no baseline elevation |
| Plants | Animal | Arthropods | Whole blood | Cells |
|
Root, Stem or Petal: 450 mg Leaf or Seed: 300 mg Fruit: 1.2 g |
HEart or Intestine: 300 mg Viscera or Brain: 240 mg Muscle: 450 mg Bones, Hair or Skin: 1g |
Insects: 6g Crustacea: 300 mg |
1 tube | 106 cells |
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3... ...
Shipment:
1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2. RNAs table tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Sample Requirements and Delivery
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Bioinformatics
For samples with an available reference genome, the BI analysis is as it follows:
Ø Raw data quality control
Ø Reference genome alignment
Ø Transcript structure analysis
Ø Expression quantification
Ø Differential expression analysis
Ø Function annotation and enrichment
For samples without a reference genome, this is the option available:
• Raw data quality control
• Assembly with structure optimization
• Differential expression analysis
• Function annotation and enrichment
Reference Genome Alignment
Data Saturation
Sample correlation and assessment of biological replicates
Differentially Expressed Genes (DEGs)
Functional Annotation of DEGs
Functional Enrichment of DEGs
Explore the advancements facilitated by BMKGene’s eukaryotic NGS mRNA sequencing services through a curated collection of publications.
Huang, L. et al. (2023) ‘Triclosan and triclocarban weaken the olfactory capacity of goldfish by constraining odorant recognition, disrupting olfactory signal transduction, and disturbing olfactory information processing’, Water Research, 233, p. 119736. doi: 10.1016/J.WATRES.2023.119736.
Jia, L. J. et al. (2023) ‘Aspergillus fumigatus hijacks human p11 to redirect fungal-containing phagosomes to non-degradative pathway’, Cell Host & Microbe, 31(3), pp. 373-388.e10. doi: 10.1016/J.CHOM.2023.02.002.
Jin, K. et al. (2022) ‘TCP Transcription Factors Involved in Shoot Development of Ma Bamboo (Dendrocalamus latiflorus Munro)’, Frontiers in Plant Science, 13, p. 884443. doi: 10.3389/FPLS.2022.884443/BIBTEX.
Wen, X. et al. (2022) ‘The Chrysanthemum lavandulifolium genome and the molecular mechanism underlying diverse capitulum types’, Horticulture Research, 9. doi: 10.1093/HR/UHAB022.
Zhang, Yujie et al. (2023) ‘A cascade nanoreactor for enhancing sonodynamic therapy on colorectal cancer via synergistic ROS augment and autophagy blockage’, Nano Today, 49, p. 101798. doi: 10.1016/J.NANTOD.2023.101798.
